Figure 1 | Cell Death & Disease

Figure 1

From: Impact of loss of BH3-only proteins on the development and treatment of MLL-fusion gene-driven AML in mice

Figure 1

Generation of murine AMLs. (a) E13.5 fetal liver cells from WT or BH3-only gene KO Ly5.2 C57BL/6 mice were infected with MLL-AF9/GFP, MLL-ENL/GFP or GFP MSCV retrovirus (Materials and methods section) and injected into sub-lethally irradiated (7.5 Gy) C57BL/6-Ly5.1 mice (2 × 106 cells/mouse). (b,c) Kaplan–Meier survival analysis of mice transplanted with fetal liver cells of the indicated genotype after infection with MLL-AF9 virus; number of recipient mice is indicated in brackets. Mice were monitored regularly and euthanized humanely when showing signs of AML-induced stress. (d) Spleen weight and (e) WBC count of individual mice at autopsy. Data points represent individual mice with mean and S.E.M. indicated. P-values were determined by unpaired t test with Welsh’s correction for differences in variance. *P<0.05, **P<0.01. Comparable data for mice transplanted with MLL-ENL virus-exposed cells is shown in Supplementary Figure S1

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