Figure 2
Forced expression of Jarid1b leads to FaDu cell differentiation. (a) K10 mRNA expression was assessed by RT-qPCR in stable Jarid1b-overexpressing (O/E) FaDu cells. (b) Protein lysates of FaDu cell-transfected Flag-Jarid1b or control were analyzed for JARID1B, Flag, K10, H3K4me3 and Histone 3(H3) by immunoblotting. (c) Correlation between Jarid1b gene expression (mRNA) and K10 gene expression (mRNA) in all human HPSCCs (n=34) based on their gene expression analysis by RT-qPCR. (d) Cell proliferation was measured by CCK8 assay at indicated time points in Jarid1b or control-overexpressing cells. *P<0.05, **P<0.01, Student’s t-test. (e) Cell proliferation was measured by CCK8 assay at indicated time points in Jarid1b or control knockdown cells. *P<0.05, Student’s t-test
