Figure 3 | Cell Death & Disease

Figure 3

From: Overexpression of JARID1B promotes differentiation via SHIP1/AKT signaling in human hypopharyngeal squamous cell carcinoma

Figure 3

β-catenin signaling is activated by overexpression of Flag-Jarid1b. (a) Puromycin selected Jarid1b-overexpressing and control FaDu cells were stained with β-catenin antibody (red) and counterstained with DAPI, showing that Jarid1b O/E cells displayed nucleus staining pattern compared with control. (b) β-catenin and its two phosphorylated isoforms, S552 and S675, were detected by immunoblotting in stable Jarid1b O/E or control cells. (c) K10 mRNA expression was analyzed by RT-qPCR upon treatment of β-catenin inhibitors, ICG001 and (d) XAV-939 and (f) AP-1 inhibitor Tan IIA at indicated concentration in stable Jarid1b O/E cells. (e) Expression of indicated AP-1 genes was assessed by RT-qPCR in stable Jarid1b O/E and control cells

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