Figure 5

Jarid1b promotes FaDu cell differentiation through directly repression of SHIP1 gene. (a) Pten and Ship1 mRNA expression were analyzed by RT-qPCR in Jarid1b O/E and control cells. (b) ChIP studies on Jarid1b-overexpressing cells showed Jarid1b binding at the SHIP1 promoter region. The scheme indicated the designed primer location at SHIP1 gene. ChIP-qPCR signal was normalized to experimental control cells infected with control vector. (c) K10 and Flag were detected by immunoblotting in control and stable Jarid1b-overexpressing FaDu cells transfected with control vector or Ship1-Flag. (d) Cell proliferation was measured by CCK8 assay at indicated time points in Jarid1b and control or Ship1-Flag co-transfected cells. Jarid1b O/E versus control ^#AP<0.01 ^#BP<0.05, Jarid1b O/E versus Jarid1b+Ship1 O/E **P<0.01, *P<0.05, Student’s t-test. (e) Hypothetic model explaining how JARID1B may contribute to HPSCC differentiation