Figure 3
Omentin promotes pulmonary endothelial barrier function after LPS insult in mice. Mice were systemically pre-treated with Ad-omentin or Ad-β-gal as a control (3 × 107 PFU total per mouse) and subjected to intratracheal injection with LPS (5 mg/kg) or PBS. Ad-omentin significantly reduced the total BALF protein concentrations (a), EBDA extravasation (b) and W/D ratios (c) in a murine model of ARDS (n=6 independent mice from each group analyzed in triplicate). (d) Western blot analysis showed that Ad-omentin significantly reversed LPS-induced reduction in the membrance protein expression of VE-cadherin and β-catenin in mouse lung tissue after LPS instillation (n=6 independent mice from each group analyzed in triplicate). The relative abundances of protein bands were quantified by measuring the corresponding band intensities; the relative values are expressed normalized to pan-cadherin signals as shown in the bar graphs. (e) Western blot analysis showed that Ad-omentin significantly reversed LPS-induced reduction in the total protein expression of VE-cadherin and β-catenin in mouse lung tissue after LPS instillation (n=6 independent mice from each group analyzed in triplicate). The relative abundances of protein bands were quantified by measuring the corresponding band intensities; the relative values are expressed normalized to GAPDH signals as shown in the bar graphs.The data are presented as the mean±S.D. *P<0.05
