Figure 2 | Cell Death & Disease

Figure 2

From: ZIKA virus elicits P53 activation and genotoxic stress in human neural progenitors similar to mutations involved in severe forms of genetic microcephaly and p53

Figure 2

Increased P53 activation in ZIKV-infected hNPCs. (a) Control (CTRL) and infected (ZIKV) NSCi90 cells were analyzed 48 h after infection by immunofluorescence with anti-total P53 (P53) and anti-flavivirus antigens (4G2), and counterstained with DAPI (4',6-diamidino-2-phenylindole). The figure shows a representative high magnification field. Note the low or absent 4G2 signal in many of the infected cells showing strong P53 nuclear positivity. (b) Quantification of P53 nuclear intensity in arbitrary units (a.u.) in cells imaged in the above experiment (n=600 for each condition). (c) Distribution of P53 nuclear intensity in non-infected cells (CTRL) and in infected cells showing undetectable (ZIKV-4G2-Neg.) or detectable (ZIKV-4G2-Pos.) levels of flavivirus antigens. Quantifications in (b) and (c) are representative of three independent infections. Scale bar=10 μm. (d) Total cell extracts of control cells and of cells infected with ZIKV for 24 or 48 h were analyzed by western blotting with the indicated antibodies. Actin, internal loading control; C-caspase, cleaved caspase. (e) Control or ZIKV-infected cells were analyzed by qRT-PCR for the indicated genes. GOI, gene of interest. Error bars, S.E.M. ***P<0.001; *P<0.05. Statistical significance was assessed by two-tailed unpaired Student's t-test in panels (c and d) and two-tailed Mann–Whitney U-test in panel (e)

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