Figure 3 | Cell Death & Disease

Figure 3

From: Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer

Figure 3

MHC class I cell surface exposure is downregulated by miR-27a in CRC cells. (a) The drawing shows the most enriched networks generated from the list of differentially expressed (DE) proteins (red elements=upregulated proteins; green elements=downregulated proteins) after miR-27a silencing using IPA. (b) Flow cytometry analysis reveals different MHC class I molecules cell surface exposure in HCT116, RKO, HT29 and their derivative clones miR27a_KD or miR27a_OE. (c) Immunofluorescence staining using an antibody against human MHC class I molecules in HCT116 CTRL and their derivative clones miR27a_KD or miR27a_OE (scale bar, 50 μm). (d) Enrichment of MHC class I molecules in the isolated plasma membrane fraction. Positivity for E-cadherin, a membrane protein, and negativity for β-Actin, a cytosolic protein, proved that the identified proteins were truly integral membrane components. The relative fold change, obtained by densitometric analysis and normalization to E-cadherin, is reported below the bands. *P≤0.05; **P≤0.01 (two-tailed Student’s t-test). Data are representative of three independent experiments and error bars represent S.D. of technical replicates (mean±S.D.)

Back to article page