Figure 5 | Cell Death & Disease

Figure 5

From: Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer

Figure 5

miR-27a acts as an oncogenic miRNA in mouse xenografts. (a) Representative photographs of the xenograft tumour masses excised from each experimental group. miR-27a inhibitor (anti-miR-27a; n=5) and scrambled control (miR-Ctrl; n=5) in HCT116 cells or miR-27a mimic (miR-27a; n=5) and scrambled control (miR-Ctrl; n=5) in HT29 cells were intratumourally injected every 7 days for four cycles starting from the day in which tumours reached the volume of 200 mm3. The growth curve of the tumours is reported for both types of xenografts. (b) Representative photomicrographs of haematoxylin and eosin staining, calreticulin and MHC class I IHC analysis and TUNEL assay, respectively, performed on paraffin-embedded sections of the same tissues; scale bars, 200 μm. (c) The histogram shows the apoptotic index as determined by TUNEL assay. (d) Western blotting analysis of the selected proteins gene identified by 2DE-DIGE performed on total protein extracts of mouse xenografts and (e) their relative quantification. Data are represented as means±S.D. from at least two independently treated tumours; *P≤0.05; **P≤0.01 (Student’s t-test)

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