Figure 6 | Cell Death & Disease

Figure 6

From: Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer

Figure 6

miR-27a upregulation in CRC samples correlates with reduced MHC class I and calreticulin expression and CD3+ and CD8+ T cells infiltration. (a) Western blotting analysis of CRT and MHC class I molecules in a representative group of normal tissues (n=5) and CRC samples (n=9) classified according to miR-27a expression; β-Actin was used as a loading control. (b) The box plots report CRT and MHC class I levels in normal versus tumour tissues (upper) and in tumour tissues according to miR-27a levels (lower). (c) IHC analysis of MHC class I, CRT, CD3+ and CD8+ T cells infiltrates in paraffin-embedded samples of normal and CRC specimens classified according to miR-27a levels (scale bar, 50 μm). (d) Box plots report CD3+ and CD8+ T cells infiltration related to miR-27a levels. P-values were calculated by paired t-test in panels (b) and (d)

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