Figure 1
From: Activation of TNFR2 sensitizes macrophages for TNFR1-mediated necroptosis
TNFR2 induces cell death in murine macrophages. (a) Hoxb8 immortalized murine MPCs and macrophages derived thereof were analyzed by flow cytometry for the indicated cell surface markers. (b) Macrophages were stimulated for the indicated times with 50 ng/ml human TNF or 200 ng/ml TNC-sc(mu)TNF80 and analyzed with respect to expression of the indicated proteins by western blotting of total cell lysates. Macrophages derived from HoxB8-immortalized MPCs of TNFR2-deficient mice served as an additional control for the TNFR2-indepenency of the observed effects. Positions of molecular weight markers (kDa) are indicated on the left. (c) MPCs and macrophages were stimulated with the indicated combinations of human TNF (50 ng/ml), TNC-sc(mu)TNF80 (200 ng/ml) and zVAD-fmk (20 μM). After 36 h, cell viability was quantified using MTT assay or by crystal violet staining. Data points derived from 6 independent experiments together with mean±S.E.M. are depicted. *p<0.05; ***p<0.001
