Figure 3
miR-134 regulates H2AX S139 phosphorylation by activating JNK. (a) The levels of p-JNK (left) and miR-134 (right) in T29 cells treated with adriamycin for 24 h or with radiation. (b) SP600125 partially abolished miR-134 upregulation induced by a 24 h of adriamycin (0.5 μM) treatment. (c) Western blot revealed that γ-H2AX levels were elevated in cells transfected with miR-134 mimics. (d) Representative western blot images of γ-H2AX and H2AX in miR-134-expressing T29 cells or control cells treated with SP600125 or DMSO. (e) miR-134 induced an increase in γ-H2AX throughout the nucleus. (f) Representative western blot images of γ-H2AX and H2AX in miR-134 inhibitors-transfected cells after a 24 h of adriamycin (0.5 μM) treatment. (g) Representative fluorescent images of γ-H2AX foci in miR-134 inhibitors-transfected cells after a 5 h of adriamycin (0.5 μM) treatment, and the number of γ-H2AX foci per nucleus are indicated. Data are shown as mean±S.D. from three independent experiments. *P<0.05 by Student’s t-test
