Figure 5
miR-134 reduces chemosensitivity in vitro and in vivo. (a) Cell proliferation assay was performed 48 h after adriamycin treatment. Survival (%) is expressed as the percentage of control cells. (b) miR-134 levels in SKOV3 and SKOV3(R) cells. (c) Cell proliferation assay in miR-134 inhibitors-transfected SKOV3(R) cells after 48 h of adriamycin treatment. (d) Cell proliferation assay of miR-con or miR-134-transfected cells treated with adriamycin or etoposide for 48 h. (e–h) Subcutaneous xenografts of SKOV3 cells infected with the miR-134 lentivirus or the control lentivirus were treated with adriamycin or 0.9% NaCl (n=5). (e) Images of the xenograft tumors. (f) The average volume of tumors. (g) The average weight of tumors. (h) The relative volume and weight of tumors treated with adriamycin or 0.9% NaCl. Data are shown as mean±S.D. from three independent experiments, unless noted otherwise. *P<0.05, **P<0.01 by Student’s t-test
