Figure 3 | Cell Death & Disease

Figure 3

From: The miR-27a-calreticulin axis affects drug-induced immunogenic cell death in human colorectal cancer cells

Figure 3

miR-27a impairs the kinetics of apoptosis execution in drug-induced ICD. (a) Western blot analysis of the apoptosis markers, PARP and caspase 3, in time-course experiments of HCT116 CRTL, miR27a_KD and miR27a_OE cells treated or not with mitoxantrone (1 μM). β-Actin was used as loading control and quantification of the cleaved protein forms is reported in (b). (c) Flow cytometry analysis of pre-apoptotic (annexin V-PE+ and 7-AAD-) and apoptotic (annexin V-PE+ and 7-AAD+) HCT116 CRTL, miR27a_KD and miR27a_OE cells treated with OXP (100 μM) in kinetics experiments. One representative experiment out of three is shown

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