Figure 7
miR-27a influences proliferation and IFN-γ production by CD4+ T cells in ex vivo experiments upon drug-mediated ICD. The CM from S-, AS- or C-transfected and treated HCT116 alone (a) or from co-cultures of the same transfected and pretreated cells cells with hu-iDCs (b) were added to naïve, freshly isolated CD4+ T cells either unstimulated or stimulated with anti-CD3/CD28 dynabeads to test their ability to proliferate. Samples were analyzed in triplicate and data are mean±S.D. and representative of three experiments. *P⩽0.05 (two-tailed Student’s t-test). (c) To analyze the production of IFN-γ, freshly isolated CD4+ T cells were cultured overnight in the presence or absence of anti-CD3/CD28 dynabeads and exposed to CMs from co-cultures as in (b). Samples were analyzed in triplicate and data are mean±S.D. and representative of three experiments. *P⩽0.05; **P⩽0.01 (two-tailed Student’s t-test)
