Figure 2

Effects of sirtinol on the expression of SIRT1 at 24 h post SAH. (a) Western blot results showed that sirtinol significantly reduced the expression of SIRT1 in both total protein and nuclear protein but had little effects on the cytoplasmic protein of SIRT1. (b) SIRT1 immunoreactivity in brain cortex was significantly decreased by sirtinol treatment. (c) SAH enhanced the expression of SIRT1 in neurons, which was significantly decreased by sirtinol treatment. Arrows point to SIRT1-positive neurons. n=6 in each group. Bars represent the mean±S.E.M. ***P<0.001, **P<0.01, *P<0.05, nsP>0.05. Scale bars=50 μm. ns, non-significant