Figure 3

Effects of SIRT1 inhibition on oxidative stress, inflammation, neuronal apoptosis, brain edema and neurological functions at 24 h post SAH. (a–d) Inhibition of SIRT1 further enhanced the increased levels of MDA, Iba-1, IL-1β, IL-6 and TNF-α after SAH. (e) Representative photomicrographs of TUNEL staining in the experimental groups. Arrows point to TUNEL-positive neurons. (f) Sirtinol treatment significantly increased the number of TUNEL-positive neurons after SAH. (g) In SAH+sirtinol group, brain water content in the cerebrum was markedly higher than that in the SAH+vehicle group. (h) When given sirtinol treatment, the impairment of neurological behaviour was more pronounced. n=6 in each group. The values are expressed as mean±S.E.M. ***P<0.001, **P<0.01, *P<0.05, ^nsP>0.05. Scale bars=50 μm