Figure 3

Pectolinarigenin inhibits osteosarcoma cells proliferation, colony formation, and induces apoptosis in osteosarcoma cell lines. (a) Osteosarcoma cells (143B, MG63.2, HOS and MG63) were treated with increasing concentrations of pectolinarigenin for 48 h and a MTS assay was performed (**P<0.01; ***P<0.001). (b) Osteosarcoma cells were seeded into six-well plates and treated with or without 10 μM pectolinarigenin for a week. Colonies were then fixed and stained with 0.1% crystal violet. Images were taken by an invert microscope (Leica). Colony numbers were counted manually (*P<0.05; **P<0.01; ***P<0.001). (c) 143B cells were treated with pectolinarigenin at the indicated doses for 48 h. Apoptotic cells were labeled with Annexin V and PI and analyzed by flow cytometry (*P<0.05; **P<0.01). (d) A western blot assay was used to detect the protein level of STAT3 target genes after pectolinarigenin exposure