Figure 2
From: p38 MAPK regulates the Wnt inhibitor Dickkopf-1 in osteotropic prostate cancer cells
Inhibition and activation of p38 MAPK signaling regulates DKK-1. (a) PC3 cells were treated for up to 3 h with small molecule inhibitors of p38 MAPK signaling; doramapimod, LY2228820 and SB202190. The most effective concentration in suppressing DKK-1 expression (10 μM) was used to assess the expression of DKK-1 mRNA in a time-dependent manner. Time points shown are in hours. (b) In PC3 cells, total DKK-1 protein and secreted protein levels were assessed for LY2228820 (LY) and SB202190 (SB) after 6 h. (c) PC3 cells were treated with the p38 MAPK signaling activator anisomycin for increasing time points from 30 min to 6 h and DKK-1 mRNA expression was assessed. All mRNA expression data of N=3 are shown as a percentage of the control untreated group and results are shown as the mean±S.D. (*P<0.05; **P<0.01, ***P<0.001)
