Figure 3
From: miR-203 inhibits the traumatic heterotopic ossification by targeting Runx2
miR-203 targets the MREs in the 3'-UTR of Runx2. (a) Sequence alignment of miR-203 with the putative MREs in the 3'-UTR of the human Runx2 gene. (b) Regulation of the Runx2 reporter gene expression by agomiR-203. (c) Dose-dependent suppression of pGL3-P1 by miR-203. (d) Effects of antagomiR-203 on the luciferase activity of pGL3-P1. (e) A schematic diagram illustrating the design of luciferase reporters with the WT-Runx2 3'-UTR and mutant Runx2 3'-UTR. (f) hFOB1.19 cells were transfected with a luciferase reporter carrying the WT or MUT 3'-UTR of the Runx2 gene (WT-Runx2-3'-UTR or MUT-Runx2-3'-UTR) and co-transfected with agomiR-203 or agomiR-NC. The effects of miR-203 on the reporter genes were determined at 48 h after transfection. The data are representative of three independent experiments. The data are shown as the means±S.D. *P<0.01; NS, not significant
