Figure 3 | Cell Death & Disease

Figure 3

From: ZNF32 contributes to the induction of multidrug resistance by regulating TGF-β receptor 2 signaling in lung adenocarcinoma

Figure 3

TGF-β signaling is essential for drug resistance induced by ZNF32 overexpression. (a) Western blot analysis of MEK/ERK signaling in PC9 cells in both the absence and presence of GEF (10 μM). (b) qRT-PCR detection of TGF-βR2 and TGF-β target gene (CDH2, TAGLN and CYR61) expression in A549 and PC9 cells. (c) Western blot analysis of TGF-βR2 expression and SMAD2 (pSMAD2) phosphorylation in PC9 cells. (d) In PC9 cells, the combination of ZNF32 overexpression and recombinant TGF-β (10 ng/ml) activates TGF-β and MEK/ERK signaling, and LY2157299 (1 μM) inhibits TGF-β and the majority of MEK/ERK signaling. (e) and (f) A 3D colony-forming assay and a flow cytometric analysis confirm that TGF-β can induce resistance in AC cells, whereas LY2157299 can counteract the effect of ZNF32 and cancel this resistance. NS, non-significant difference. Each column and bar represents the mean±S.D. of three independent experiments. The photograph shows a representative result from three independent experiments

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