Figure 6
ZNF32 is positively correlated with TGF-βR2 expression and negatively correlated with prognosis. (a) IHC detection of the expression of ZNF32 and its downstream target TGF-βR2 in AC samples (left panel). A correlation analysis demonstrates that ZNF32 expression is positively correlated with TGF-βR2 expression in AC tissues (right panel). (b) Kaplan–Meier survival curve for 52 patients who were categorized into two groups based on the nuclear ZNF32 IHC score. (c) Kaplan–Meier survival curve for 37 patients who had received CIS-based chemotherapy, whose responses to chemotherapy are known, and who were categorized into two groups based on the nuclear ZNF32 IHC score. (d) Multivariate analysis of ZNF32 expression in patients. The risk ratio (proportional hazard) was calculated with respect to the following parameters: ±nuclear ZNF32 expression, stage (I/II–IIIA), grade (high/low), Ki67 (high/low), and sex (male/female). (e) Comparison of ZNF32 expression between patients with and without brain metastasis. (f) Tissue slices were cultured with CIS (20 μM) for 3 days, and IHC was then performed for the comparison of Ki-67 expression and TUNEL positivity between the ZNF32high and ZNF32low groups (n=5 per group). NS, non-significant difference. Each column and bar represents the median±S.D. of three independent experiments. The photograph shows a representative result from three independent experiments
