Figure 1 | Cell Death & Disease

Figure 1

From: Metformin protects against apoptosis and senescence in nucleus pulposus cells and ameliorates disc degeneration in vivo

Figure 1

Metformin treatment inhibits TBHP-induced nucleus pulposus cell apoptosis and senescence. (a) Cell Counting Kit-8 (CCK-8) results of nucleus pulposus cells treated with different concentrations of metformin for 24 h. (b) CCK-8 results of nucleus pulposus cells treated with different concentrations of TBHP for 4 h. (c) CCK-8 results of metformin-pretreated nucleus pulposus cells induced by TBHP. (d) Nucleus pulposus cells were pretreated with metformin and then TBHP and imaged by phase-contrast microscopy (original magnification × 100, scale bar: 50 μm). (e–g) Protein content of cleaved caspase3, p16INK4a of nucleus pulposus cells treated with TBHP and TBHP plus metformin. (h) The mRNA expression of Col2a1 of nucleus pulposus cells treated with TBHP and TBHP plus metformin was measured by real-time PCR. The data in the figures represent the averages±S.D. Significant differences between the treatment and control groups are indicated as **P<0.01,*P<0.05, n=3

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