Figure 3 | Cell Death & Disease

Figure 3

From: Metformin protects against apoptosis and senescence in nucleus pulposus cells and ameliorates disc degeneration in vivo

Figure 3

Inhibition of AMPK by siRNA significantly attenuates metformin-induced autophagy in nucleus pulposus cells. Cells were transfected with negative control siRNA (con-siRNA) or AMPK-siRNA before receiving metformin(100 μM). (a–c) The protein expression of p-AMPK, AMPK, LC3, Beclin-1 and p62 in the nucleus pulposus cells as treated above. (d, e)The representative LC3-positive autophagic vesicles were detected by immunofluorescence staining combined with DAPI staining for nuclei (scale bar: 25 μm). The data in the figures represent the averages±S.D. Significant differences between the treatment and control groups are indicated as **P<0.01, *P<0.05, n=3.

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