Figure 3 | Cell Death & Disease

Figure 3

From: The protein level and transcription activity of activating transcription factor 1 is regulated by prolyl isomerase Pin1 in nasopharyngeal carcinoma progression

Figure 3

Pin1 stabilizes the expression of ATF1 in post-transcription level. (a) Western blotting displayed a positive correlation between Pin1 and ATF1 in a panel of NPC cell lines and NPC tissues. CI, chronic inflammation of nasopharynx tissue; NAT, normal adjacent tissue of NPC. (b) qRT-PCR assay showed the mRNA level of ATF1 was not regulated by upregulation of Pin1 using CNE1 cells transfected with Pin1 plasmid for 24 h. (c) qRT-PCR assay showed the mRNA level of ATF1 was not affected by downregulation of Pin1 using CNE2 cells transfected with shPin1 plasmid for 48 h. (d) Pin1+/+ and Pin1−/− mouse embryonic fibroblasts cells were treated with 50 μg/ml CHX for indicated durations followed by WB analysis. The quantitative data of ATF1 protein are represented in the right panel. (e) Stable Pin1 expression cells and control CNE1 cells were treated with 50 μg/ml CHX for indicated durations followed by WB analysis. The quantitative data of ATF1 protein are represented in the right panel. (f) Stable Pin1 knockdown cells and control CNE2 cells were treated with 50 μg/ml CHX for indicated durations followed by WB analysis. The quantitative data of ATF1 protein are represented in the right panel. 'shNC' stands for the negative control of shRNA

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