Figure 4 | Cell Death & Disease

Figure 4

From: The protein level and transcription activity of activating transcription factor 1 is regulated by prolyl isomerase Pin1 in nasopharyngeal carcinoma progression

Figure 4

Pin1 regulates the transcriptional activity of ATF1 in NPC. (a and b) Luciferase assays showed Bcl-2 promoter activity regulated by various modifications of ATF1 and Pin1. (c and d) qRT-PCR assays showed Bcl-2 mRNA regulated by various modifications of ATF1 and Pin1. (e and f) Western blotting assays showed Bcl-2 protein regulated by various modifications of ATF1 and Pin1. CNE1 cells were transfected with overexpression plasmids for 24 h and with shPin1 plasmid for 48 h. In panels (a, c and e), vector was added in 'Vec+ATF1' to make this transfection system equal to 'Pin1+ATF1' (ATF1 plasmid was equal in the two transfection; whereas vector was used to replace Pin1 plasmid.). *P<0.05

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