Figure 2

Effect of Ccr2 knockout on ONL thinning induced by exposure to chronic blue light in mice. Representative retinal images of H&E staining in the (a and e) normal WT Ctrl mice, (b and f) WT+BLE mice (500 lux), (c and g) Ccr2^−/− Ctrl mice, and (d and h) Ccr2^−/−+BLE mice (500 lux). Panels (a–d) are retinal segments between optic nerve and approximately 1000 μm from the optic nerve with a scale bar of 50 μm. Panels (e and f) are retinal segments between 400 and 600 μm away from optic nerve in the superior area with a scale bar of 20 μm (green box in panels (a–d)). (i) Measurement of thickness of the ONL in the four groups after 1 month. (j) Measurement of thickness of the ONL in the four groups after 3 months. Data are shown as mean±S.E.M., n=6. Significant differences were calculated using one-way analysis of variance followed by Tukey’s multiple comparison test. *P<0.05, **P<0.001 compared with Ccr2^−/−+BLE mice