Figure 3

MSCs switch the mitochondrial metabolism of T-ALL cells toward the glycolytic phenotype. (a and b) The glucose uptake and lactate production capacity were compared in T-ALL cells cultured alone or with MSCs in the Transwell or direct coculture systems. (c) Intracellular NADH production and the NAD+/NADH ratio were determined in T-ALL cells subjected to the three culture models. (d and e) Cellular ATP levels and MMP were most strongly decreased in T-ALL cells directly cocultured with MSCs, compared with cells mono-cultured in suspension. All data above are represented as the mean±S.E.M. of three independent experiments (*P<0.05; **P<0.01; t-test)