Figure 4

Estrogen promotes the differentiation of Treg cells through elevated IDO1 levels in the ectopic lesion. (a) Flow cytometric analysis was used to determine the percentage of Treg cells in the co-culture system obtained with estrogen, estrogen plus 1-MT, or neither. Numbers in quadrants indicate the percentage of cells. (b) Quantification of Treg cells shown in a. Values indicate mean±S.D., n=12, *P<0.05, ***P<0.001, two-tailed, paired t-test. Co, the naive T cells-macrophage-endometrial stromal cell (ESC) co-culture system. E2, estrogen