Figure 4 | Cell Death & Disease

Figure 4

From: Systemic application of 3-methyladenine markedly inhibited atherosclerotic lesion in ApoE−/− mice by modulating autophagy, foam cell formation and immune-negative molecules

Figure 4

3-MA decreased foam cell macrophage formation in vitro and accumulation of lipid droplets within atherosclerotic plaque. (a) Representative images of BODIPY (green) and LC3 (red) in aortic plaque from control mice and 3-MA-treated mice. Red boxes highlight LC3-positive area and yellow boxes LC3-negative areas. Scale bar, 50 μm. (b) Cells were preincubated with 3-MA for 30 min, then stimulated with 25 μg/ml oxLDL for foam cell induction. Oil Red O staining was performed at different times after oxLDL stimulation. The proportion of Oil Red O-positive cells from three independent experiments was calculated and data are mean±S.D. **P<0.01, ***P<0.001, unpaired two-tailed Student’s t-test

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