Figure 2

GRK6 knockout reduces BM HSC and progenitor populations. (a and b) Representative photograph of WT and GRK6^−/− tibia (a) and statistics of total cell number from two femurs and two tibiae of mice (n=6–7 mice each group) (b). (c) Femoral sections were subjected to HE staining (left, 40 × original magnification and middle, 400 × original magnification) and bone marrow smears were subjected to Wright-Giemsa’s staining (right, 1000 × original magnification). Arrows indicate abnormal cells. Bars represent 500 μm (left) or 20 μm (middle and right). (d–l) Representative flow cytometric data (d) and (e–j) statistics of bone marrow LSK (Lin⁻Sca-1⁺cKit^hi) (e), SP (LSKHoechst 33342^lo) (f), LT-HSC (LSKFlt3⁻CD34⁻) (g), ST-HSC (LSKFlt3⁻CD34⁺) (h) and MPP (LSKFlt3⁺CD34⁺) (i), lymphoid competent HSC (LSKCD48⁻CD150^lo) and myeloid competent HSC (LSKCD48⁻CD150^hi) (j), CLP (Lin⁻Sca-1⁺cKit⁺IL7Rα⁺FcγRII/III⁻) (k), CMP (Lin⁻Sca-1⁻cKit⁺FcγRII/III^loCD34⁺), GMP (Lin⁻Sca-1⁻cKit⁺FcγRII/III^hiCD34^hi) and MEP (Lin⁻Sca-1⁻cKit⁺FcγRII/III^loCD34⁻) (l) populations of 8–12 weeks old WT and GRK6^−/− mice (n=4–10 each). *P<0.05, **P<0.01, ***P<0.001 by two-tailed, unpaired Student’s t-test. Data are expressed as mean±S.E.M. CMP, common myeloid progenitors; GMP, granulocyte/monocyte progenitors; LT-HSC, long-term HSC; MEP, megakaryocyte/erythrocyte progenitors; MPP, multi-potent progenitors; ST-HSC, short-term HSC