Figure 4

GRK6 regulates lymphoid differentiation. (a–d) Single-dose 5-FU assay for proliferation. (a) GRK6^−/− mice and WT littermates were intraperitoneally injected with a single dose of 5-FU on day 0 followed by serial samplings of peripheral blood at day 4, 8, 14 (n=3–7 each). Peripheral blood count of (b) WBCs, (c) lymphocytes and (d) neutrophils were determined with hematology analyzer and presented as percentage of original cell count. Two-way ANOVA with Bonferroni post hoc, *P<0.05, ***P<0.001, on the right, within genotype; on top, within each day. Data are expressed as mean±S.E.M. (e,f) Whole bone marrow culture to test clonogenic ability with (e) CFU-Pre-B (n=3 each with duplicate) or (f) CFU-C assay (n=5 each with duplicate). On top, ^###P<0.001 of total colony number in (f); *P<0.05, ***P<0.001 WT versus GRK6^−/− of each population by two-tailed, unpaired Student’s t-test. Data are expressed as mean±S.E.M. 5-FU, 5-Fluoruracil