Figure 6
From: GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal
GRK6 regulates DNA-PKcs phosphorylation. (a) IP–MS of GRK6 in 231 cell line. Numbers indicate bands that were excised and subjected to mass spectrometry analysis. (b) GRK6 interacts with DNA-PKcs. HEK293T cells were transfected with FLAG-GRK6 or FLAG-GFP, and immunoprecipitated with anti-FLAG M2 beads. (c) H2O2 stimulation of Jurkat T cells infected with control (shLacZ) or GRK6 knockdown (shGRK6) lentivirus. Infected cells were serum starved for 8 h, treated with 200 μM H2O2 and collected at 0, 30, 60, 180 min afterwards. *GRK5 band recognized by GRK4-6 antibody. (d) Densitymetric analysis of DNA-PKcs, pDNA-PKcs, ATM, pATM, γH2AX and GRK6 (n=3). Expression level were normalized to β-ACTIN and shown as relative level to LacZ-0 min. Two-way ANOVA with Bonferroni post hoc; *P<0.05, **P<0.01, ***P<0.001, within genotype; #P<0.05, ###P<0.001, Bonferroni post hoc within treatment (versus LA dose 0). Data are expressed as mean±S.E.M.
