Figure 4
From: MDM2 prevents spontaneous tubular epithelial cell death and acute kidney injury
Tubular injury in tubular cell-specific MDM2 knockout mice kidneys. (a) Representative images of PAS-stained kidney sections at a magnification 200x from Pax8rtTA-cre;MDM2f/f and control mice treated for 4, 8 or 11 days with doxycycline showing the progressive aggravation of tubular injury. At day 4 was no tubular damage apparent, at day 8 the tubular cells swelling and vacuolization were the prominent pathologic features and at day 11 the global tubular damage with massive cell loss, tubular dilation and tubular casts was detected. Tubular injury was quantified on PAS-stained renal section as described in Materials and methods. (b) Lotus tetragonolobus lectin staining identified proximal tubuli, Tamm-Horsfall protein (THP) staining identified distal tubuli and Aquaporin 2 identified the collecting ducts in Pax8rtTA-cre;MDM2f/f and control mice kidneys treated for 4, 8 or 11 days with doxycycline. The quantitative assessment of tubuli with intact staining patterns is shown for each staining. Data are means±S.E.M. from six mice in each group. *P<0.05, **P<0.01, ***P<0.005. All images are shown at a magnification of × 100
