Figure 7
From: MDM2 prevents spontaneous tubular epithelial cell death and acute kidney injury
Tubular epithelium compensates only partially for the cell loss caused by MDM2 depletion. To induce a sub-lethal deletion of MDM2 in tubular epithelial cells in our mouse kidney model we treated Pax8rtTA-cre;MDM2f/f mice intermittently with 2 mg/ml doxycycline for 4 weeks. (a) Pax8rtTA-cre;MDM2f/f mice treated with intermittent regimen of doxycycline had elevated plasma creatinine and plasma BUN at 4 weeks (n=5–6 mice in each group). (b) Representative images of kidney sections from control MDM2f/f and Pax8rtTA-cre;MDM2f/f mice treated for 4 weeks intermittently with doxycycline and stained with PAS, Masson trichrome and p53 antibody. All images are shown at a magnification of × 200. (c) Real-time PCR analysis of pro-fibrotic genes mRNA prepared from kidneys isolated from experimental and control mice treated for 4 weeks with doxycycline. Data are means±S.E.M. *P<0.05, **P<0.01, ***P<0.005
