Table 1 Primers used for real-time RT-PCR
From: MDM2 prevents spontaneous tubular epithelial cell death and acute kidney injury
Target | Primer sequence | |
|---|---|---|
MDM2 | Forward primer | 5′-TGTGAAGGAGCACAGGAAAA -3′ |
Reverse primer | 5′-TCCTTCAGATCACTCCCACC -3′ | |
p53 | Forward primer | 5′-TCCGACTGTGACTCCTCCAT-3′ |
Reverse primer | 5′-CTAGCATTCAGGCCCTCATC-3′ | |
KIM-1 | Forward primer | 5′-TGGTTGCCTTCCGTGTCTCT-3′ |
Reverse primer | 5′-TCAGCTCGGGAATGCACAA-3′ | |
NGAL | Forward primer | 5′-TGAACTTCTGAAAACGGCT-3′ |
Reverse primer | 5′-AGCAGCAAGGGCACAAT-3′ | |
TIMP-2 | Forward primer | 5′-CGTTTTGCAATGCAGACGTA-3′ |
Reverse primer | 5′-GAATCCTCTTGATGGGGTTG-3′ | |
TGF-β | Forward primer | 5′-GGAGAGCCCTGGATACCAAC-3′ |
Reverse primer | 5′-CAACCCAGGTCCTTCCTAAA-3′ | |
Fibronectin | Forward primer | 5′-GGAGTGGCACTGTCAACCTC-3′ |
Reverse primer | 5′-ACTGGATGGGGTGGGAAT-3′ | |
Collagen1a1 | Forward primer | 5′-ACATGTTCAGCTTTGTGGACC-3′ |
Reverse primer | 5′-TAGGCCATTGTGTATGCAGC-3′ | |
Collagen4a1 | Forward primer | 5′-GTCTGGCTTCTGCTGCTCTT-3′ |
Reverse primer | 5′-CACATTTTCCACAGCCAGAG-3′ | |
18s | Forward primer | 5′-GCAATTATTCCCCATGAACG-3′ |
Reverse primer | 5′-AGGGCCTCACTAAACCATCC- 3′ |
