Figure 4

JEV RNA interacts with GRP78, PHB and hnRNPC. (a) Viral amplification is visualized only in the samples that were conjugated to GRP78, PHB and hnRNPC antibodies. PC represents positive control of JEV infection. The Co-IP bands appeared in same positions (˜400 bp) with positive control bands implying JEV RNA binding with these proteins. (b) Sequencing data (representative image of one of the three Co-IP experiments) reveal more than 95% similarity of the identified band with JEV GP78 genome. (c) GRP78 is secreted in hNS1 media post 72 h of infection and later found in progressive time points, ponceau profile of the SDS-PAGE gels indicate equal protein loading in respective experiments. (d) Heat shock protein chaperones were also found in hNS1 secretion at progressive time points of JEV infection. (C1, C2, C3 and J1, J2, J3 represent 72 h time point data of three independent experiments; C, J24, J48 and J72 indicate progressive time point data of 24, 48 and 72 h of JEV infection). All data are representative of three independent experiments