Figure 5 | Cell Death & Disease

Figure 5

From: Japanese encephalitis virus induces human neural stem/progenitor cell death by elevating GRP78, PHB and hnRNPC through ER stress

Figure 5

siRNA knockdown of GRP78, PHB and hnRNPC affects mature virion release. (a, b) siRNA treatment of GRP78, PHB and hnRNPC before viral intervention reduces caspase 3 activation in hNS1 cells. (aA, bA) Immunoblots show reduced PHB and hnRNPC expression following GRP78 ablation. (aB, bB) Reduced expression of GRP78 and hnRNPC were observed in case of PHB ablation. (aC, bC) No significant changes in GRP78 and PHB expression were visualized in case of hnRNPC knockdown. (c) In all ablation studies, comprehensible depletion in mature virion release was observed (viral band detected at 400 bp, 1 kb DNA ladder was used) (*P<0.05, #P<0.01). Data are representative of three independent experiments (mean±S.D.)

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