Table 1 Proteins showing differential expression after JEV infection, identified by MS/MS analysis of gel excised spots

From: Japanese encephalitis virus induces human neural stem/progenitor cell death by elevating GRP78, PHB and hnRNPC through ER stress

Spot No

Protein ID a

Matched peptides

% coverage

Mowse score b

MW (theor/obs)

pI

1

Uncharacterized protein C19orf45 (Homo sapiens) NP_940936

K.TTMGSDYCPSEWR.Q

K.APNLHLQQSYLPR.G

K.DEFPFKYQGPAALR.L

14

72

57979/50000

9.04

2

Lamin isoform D (Homo sapiens) NP_001244303

R.VAVEEVDEEGKFVR.L

R.EFESRLADALQELR.A

R.IRIDSLSAQLSQLQK.Q

17

84

64253/65000

6.30

3

Prohibitin (Homo sapiens) NP_001268425

R.FDAGELITQR.E

K.DLQNVNITLR.I

R.ILFRPVASQLPR.I

26

82

29859/25000

5.57

4

Mitochondrial ATP synthase, H+ transporting F1 complex beta subunit (Homo sapiens) NP_001677

R.LVLEVAQHLGESTVR.T

R.IMDPNIVGSEHYDVAR.G

R.AIAELGIYPAVDPLDSTSR.I

28

94

48083/51000

4.95

5

Calreticulin precursor (Homo sapiens) NP_004334

K.KVHVIFNYK.G

K.EQFLDGDGWTSR.W

K.IKDPDASKPEDWDER.A

13

72

48283/52000

4.29

6

78 kDa glucose-regulated protein precursor (Homo sapiens). NP_005338

R.IINEPTAAAIAYGLDKR.E

K.VTHAVVTVPAYFNDAQR.Q

R.IEIESFYEGEDFSETLTR.A

24

153

72402/75000

5.07

7

Vimentin (Homo sapiens) NP_003371

K.FADLSEAANRNNDALR.Q

R.ETNLDSLPLVDTHSKR.T

R.ISLPLPNFSSLNLR.E

40

160

53676/60000

5.06

8

Heterogeneous nuclear ribonucleoprotein C (C1/C2) (Homo sapiens) NP_001070911

R.MYSYPAR.

R.VPPPPPIAR.A

K.GFAFVQYVNER.N

27

72

19079/30000

10.22

9

Zinc finger protein 224 (Homo sapiens) NP_037530

K.IWMMKTAIQR.E

R.LNLDMHQRVHMGEK.T

R.VHSGEKPFKCEECGK.G

16

67

84881/40000

8.96

10

RNA polymerase II subunit A C-terminal domain phosphatase. (Homo sapiens) NP_430255

R.SMEAHNILSK.R

K.DLFDLILTCEER.

K.TTYDQMYNDLLRK.D

25

56

23016/100000

5.16

11

Actin, cytoplasmic 1 (Homo sapiens) NP_001092

K.IWHHTFYNELR.V

K.QEYDESGPSIVHR.K

K.SYELPDGQVITIGNER.F

20

74

38950/40000

5.19

12

HYOU1 protein (Homo sapiens) NP_001124463

K.QADNPHVALYQAR.F

R.FPEHELTFDPQR.Q

R.DAVVYPILVEFTR.E

11

73

73229/100000

5.74

13

Nebulin-related anchoring protein (Homo sapiens) NP_932326

R.ANAANLSEVKYK.E

K.KAYGLQSELQYK.A

K.GSFPAMITPAYQR.A

6

69

193924/40000

9.22

  1. aNCBI accession number of identified proteins is mentioned.
  2. bMS/MS data of three peptides for each spot was searched against NCBI database in the taxonomy group of Homo sapiens using Mascot tool. A total of three peptides were subjected to MS/MS analysis and the fragment ion data were searched against the data base.
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