Figure 1 | Cell Death & Disease

Figure 1

From: Distinct outcomes of CRL–Nedd8 pathway inhibition reveal cancer cell plasticity

Figure 1

MLN effect on prostate cancer cell lines. (a) Plot of cellular ATP versus MLN concentration (mean±S.D.). Microscopy images on the right show the changes in cell morphology at 500 nM MLN. Scale bar, 100 μm. (b) Apoptosis induction by MLN shown as percentage of apoptotic cells (mean±S.D.). Fluorescence microscopy images on the right show Hoechst (DNA, blue) and CellEvent (CE, green) staining. Scale bar, 50 μm. (c) Analysis of Nedd8 conjugates in VCaP cells by western blotting with Nedd8-specific antibody and anti-GAPDH for loading control. The sizes of bands 1–6 correspond to neddylated cullins (b2/3), NAE1 (b4), UBA3 (b5), and Ube2M (b6). (d) The abundance of Nedd8 species was quantified by ImageJ using (c), and normalized first to GAPDH, then to vehicle control. (e) Immunofluorescence analysis of Nedd8 conjugates in VCaP cells with Nedd8-specific antibody (NEDD8, green) and Hoechst dye (DNA, red). Scale bar, 10 μm

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