Figure 3
From: Distinct outcomes of CRL–Nedd8 pathway inhibition reveal cancer cell plasticity
MLN induces reversible growth arrest in spheroid model. (a) Effect of MLN on spheroid growth analyzed by phase-contrast microscopy. An × 10 objective was used, except for control spheroids, for which starting from day 46, an × 4 objective was used. On day 40, spheroids cultured with 50 nM MLN were transferred into MLN-free medium. Scale bars, 200 μm. (b) Quantitative analysis of spheroid growth based on microscopy images (mean±S.D.). (c) Induction of apoptosis and senescence by MLN. Top phase-contrast/fluorescent microscopy images show Caspase-3/7 activity measured with CellEvent. Bottom panels show senescence-associated beta-galactosidase activity (β-GAL) measured with X-GAL. Scale bar, 100 μm. See also Supplementary Figures S5–S8
