Figure 6
From: Distinct outcomes of CRL–Nedd8 pathway inhibition reveal cancer cell plasticity
Opposite roles of AR and ERG in MLN-induced cell fate. (a) Effect of MLN on AR and AR-dependent proteins. Western blotting was performed with protein-specific antibodies. The protein level was normalized first to GAPDH, then to vehicle control. (b) MLNlow stimulates the secretion of PSA by VCaP cells and spheroids as assessed by ELISA. (c) Effect of MLN on the expression of AR target genes measured by quantitative RT-PCR (Supplementary Table S4). Data are normalized to 18 S rRNA expression and to vehicle control (mean±S.D.). (d) Opposite effects of AR and ERG knockdowns on MLN-induced apoptosis. VCaP cells were transfected with indicated siRNAs and treated with MLN (see also Supplementary Figure S14). (e) Changes in protein expression induced by AR and ERG knockdowns analyzed by Western blotting with protein-specific antibodies. Asterisk indicates active c-Myc isoform. The protein level was normalized first to GAPDH, then to siCTL
