Figure 4

Lower TRAP1 expression is associated with more advanced disease and EMT. (a) Correlation between TRAP1 mRNA expression and tumor sample site from the Tothill data set (P-values determined by the Wilcoxon rank sum test). (b) Real-time RT-PCR analysis of SNAIL, SNAI2/SLUG, SNAIL3, MMP9, MMP2 and E-Cadherin expression in PEA1 cells 72 h after transfection with non-targeted control siRNA or TRAP1-directed siRNA. Data are expressed as mean±S.E.M. from three independent experiments with technical triplicate each. Numbers above bars indicate the statistical significance (P-value), based on one-sample t-test. Dashed line indicate expression level of the relative control siRNA-transfected cells. (c) Correlation between TRAP1 mRNA expression and E-CADHERIN protein expression. Statistical analysis by Spearman's rank correlation coefficient. (d–f) Correlation between TRAP1 mRNA expression and SLUG/SNAI2 (d), MMP2 (e) and MMP9 (f) mRNA expression. Statistical analysis by Pearson's product moment correlation coefficient. (g) Total lysates obtained from PEA1 cells transfected for 72 h with non-targeted control siRNA or TRAP1-directed siRNA were separated by SDS-PAGE and immunoblotted with the indicated antibodies. Numbers indicate densitometric band intensities, calculated by assuming protein levels of the control equal 1. Images are representative of three independent experiments