Figure 5
From: Inflammatory macrophages can transdifferentiate into myofibroblasts during renal fibrosis
Macrophages undergoing transition have a predominant M2 phenotype in the UUO kidney. (a) Three-color confocal microscopy identifies α-SMA+F4/80+CD206+ triple-stained M2-type cells (arrows) in an area of interstitial fibrosis in day 7 UUO mouse kidney. (b) Quantitative analysis of single-, double- and triple-stained cell populations from the immunofluorescence staining for day 7 UUO mouse kidney (n=6). (c) Three-color flow cytometric analysis of cells isolated from day 7 UUO kidney. Approximately 50% of F4/80+ cells co-express CD206 in the UUO kidney. However, gating on α-SMA+F4/80+ double-stained cells identifies that approximately 80% of these cells express CD206, indicating enrichment of M2-type macrophages in this population. Scale bar, 20 μM
