Figure 4
From: Bcl-3 regulates TGFβ signaling by stabilizing Smad3 during breast cancer pulmonary metastasis
Bcl-3 regulates TGFβ target gene expression. (a) qRT-PCR analysis of metastasis-related genes in Bcl-3 knockdown LM2 cells. (b) Gene ontology analysis shows altered signaling pathway. (c) Heat map of the altered genes shared between the four profiles; red shows induced and green shows repressed genes, log2-based scale. MDA-MB-231 cell RNA samples were collected after TGFβ stimulation for 24 h. (d) Venn diagram representation of differentially expressed genes in the two groups: genes affected by TGFβ stimulation and genes affected by Bcl-3 depletion after TGFβ stimulation. (e) qRT-PCR analysis for ANGPTL4 in the indicated MDA-MB-231 and LM2 cells with or without TGFβ stimulation for 24 h. The results are the means±S.E.M.s for each cohort (n=3). Student’s t-test was used. P<0.05. (f) Immunoblots for Bcl-3, Snail, ID1 and ID3 in the indicated MDA-MB-231 cells under TGFβ stimulation. (g) Wound-healing assay carried out on the indicated MDA-MB-231 cells under TGFβ stimulation, scale bar=50 μm
