Figure 1
From: Sensitivity of hematopoietic stem cells to mitochondrial dysfunction by SdhD gene deletion
Analysis of leukocytes in bone marrow of SDHD-ESR mice. Bone marrow cell preparations were labeled with antibodies against the common leukocyte antigen, CD45; granulocyte- and monocyte-specific, CD11b antigen; and T-cell-specific CD3 antigen, for the detection by flow cytometry. (a) Representative dot-plots of total BM cells labeled with CD45, CD11b, and CD3 markers from SdhDflox/+ (+/+) and SdhDflox/− CRE (SDHD-ESR) individuals. For simplicity, the SdhDflox/− (+/−) animal is omitted as it displays the same phenotype as the +/+ control. Populations are also distinguishable by colors. SSC, side-scattered component. (b) Quantification of CD45+ leukocytes relative to the total number of events, and granulocyte/macrophage and T cells relative to total CD45+ cells. Each symbol represents data from a single animal. (c) Percentage of Annexin V+ events in CD45+ population. N=7–17 per genotype. (d) Relative SdhD mRNA levels in granulocytes and monocytes/macrophages (CD11b+) and T cells (CD3+). N=6–11 per genotype. Bars represent the mean values±S.E.M. Statistical significance: *P⩽0.05; **P⩽0.01; ***P⩽0.001. a.u., arbitrary units
