Figure 2

Analysis of B cells in bone marrow of SDHD-ESR mice. Bone marrow cell preparations were labeled with an antibody against the B-cell-specific antigens, B220 and IgM. (a) Representative flow cytometry dot-plots of CD45⁺ BM cells labeled with anti-B220 marker from +/+ and SDHD-ESR individuals. Two distinct B220⁺ populations, enclosed in rectangles, can be distinguished based on its high (B220^high) or low (B220^low) expression of the marker. (b) Quantification of B220^low and B220^high cells relative to the total number of CD45⁺ events. (c) Number of colony-forming units from B-cell precursors (CFU-preB) per 10⁵ cells seeded. Each dot is the average result of two different cultures from each animal. (d) Relative SdhD mRNA levels in total B220⁺ population N=3–11 per genotype. (e) Representative flow cytometry dot-plots of total BM cells expressing B220 and IgM markers from +/+ and SDHD-ESR individuals. Three distinct B220⁺ populations can be distinguished based on IgM and high (B220^high) or low (B220^low) B220 expression of the marker corresponding to three stages of maturation: pro-B-cell (IgM⁻ B220^low), intermediate precursor (IgM⁺ B220^low) and mature B-cell (IgM⁺ B220^high). (f) Quantification of number of cells in each maturation stage relative to the total number of cells. (g) Percentage of Annexin V⁺ events in each maturation stage. N=4–10 per genotype. Bars represent the mean values±S.E.M. Statistical significance: *P⩽0.05; **P⩽0.01; ***P⩽0.001