Figure 3
From: Sensitivity of hematopoietic stem cells to mitochondrial dysfunction by SdhD gene deletion
Analysis of T cells in thymus of SDHD-ESR mice at different maturation stages. (a) Photographs of thymi (Th) from +/+ and SDHD-ESR individuals at 2 and 5 weeks after tamoxifen treatment. Note the complete disappearance of the mutant organ after 5 weeks. (b) Relative SdhD mRNA levels in the whole organ. N=3–5 per genotype and group. (c) Quantification of T cells (CD3+) relative to the total number of events. (d) Quantification of undifferentiated thymocytes (Lin− CD3−) relative to the total number of events. (e) Percentages of cells at different maturation stages, from double-negative (DN)1 to DN4, within the Lin− CD3− fraction. DN1 to DN4 were defined by the expression of CD44 and CD25 markers (more details in the text). N=3–7 per genotype. Bars represent the mean values±S.E.M. Statistical significance: **P⩽0.01
