Figure 4
From: Sensitivity of hematopoietic stem cells to mitochondrial dysfunction by SdhD gene deletion
Analysis of hematopoietic stem (HSC) and progenitor (HPC) cells in bone marrow of SDHD-ESR mice. The lineage negative (Lin−) cell fraction was identified as described in the ‘Materials and Methods’ section. The Lin− cells were labeled with antibodies against c-Kit, Sca1, CD34, CD16/32, and Flt3 antigens for detection by flow cyometry. (a) Representative dot-plots of Lin− fractions from +/+ and SDHD-ESR individuals. Rectangles indicate Lin− c-Kit+ Sca1− (LK) and Lin− c-Kit+ Sca1+ (LKS) populations, also distinguishable by colors. (b) Diagram showing the cell types differentiated within LKS and LK populations as well as their immunophenotypes based on the expression of CD34, Flt3, and CD16/32 markers. (c) Quantification of LK and LKS cells relative to the total number of Lin− events. (d) Relative SdhD mRNA levels in each subpopulation of the Lin− fraction. N=5–16 per genotype and population. (e) Percentage of Annexin V+ events in each subpopulation. N=3–9 per genotype and population. Quantification of subpopulations contained in LK (f) and LKS (g) fractions relative to the total number of Lin− events by flow cytometry with the corresponding antibodies (see the ‘Materials and Methods’ section). CLP, common lymphoid progenitors; CMP, common myeloid progenitor; GMP, granulocyte/monocyte progenitor; LT-HSC, long-term HSC; MEP, megakaryocyte/erythroid progenitor; MPP, multipotent progenitors; ST-HSC, short-term HSC. Bars represent the mean values±S.E.M. Statistical significance: *P⩽0.05; **P⩽0.01; ***P⩽0.001
