Figure 7
From: Globozoospermia and lack of acrosome formation in GM130-deficient mice
Co-localization of TGN46 and AP1 was disrupted in 2 months GM130-deficient spermatids. The expression of AP1 and TGN46 was examined by immunofluorescence. TGN46 (d, red) and AP1 (e, green) proteins were detected in the germ cells of control testes, and these two proteins were completely co-localized (f, inset, white arrow). TGN46 (a, red) and AP1 (b, green) proteins were also detected in the GM130-deficient germ cells, whereas the TGN46 and AP1-positive punctae were substantially smaller than those in the control germ cells, and a substantial portion of the AP1-positive punctae were not co-localized with TGN46 (c, inset, white arrow)
