Figure 6
From: WISP-1 positively regulates angiogenesis by controlling VEGF-A expression in human osteosarcoma
Knockdown of WISP-1 decreases angiogenesis in vivo. (a) Chick embryos were incubated with osteosarcoma 0% DMEM (control), osteosarcoma CM, or VEGF-A (50 ng/ml) for 4 days, then photographed with a stereomicroscope (bar=1 mm) and quantified by vessel count. (b–d) Mice were injected subcutaneously with Matrigel mixed with 0% DMEM (control) or osteosarcoma CM for 7 days, then the plugs were excised, photographed, stained with CD31 (bar=50 μm) and quantified for hemoglobin content. Each experiment was performed in triplicate (N=3). Results are expressed as the mean±S.E.M. *P<0.05 compared with control; #P<0.05 compared with the control-shRNA group
